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KMID : 0379120100380020112
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Korean Journal of Mycology
2010 Volume.38 No. 2 p.112 ~ p.119
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Comparative Molecular Phylogenetic Relationships in Different Strains of Pleurotus spp.
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Cho Hae-Jin
Lee Jae-Seong
Yoon Ki-Nam
Nuhu Alam
Lee Kyung-Rim
Shim Mi-Ja
Lee Min-Woong
Cheong Jong-Chun
Shin Pyung-Gyun
Yoo Young-Bok
Lee U-Youn
Lee Tea-Soo
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Abstract
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Pleurotus spp. have been used for edible and medicinal purposes in Asian countries for a long time. The fruiting bodies of the Pleurotus ostreatus, Pleurotus citrinopileatus and Pleurotus salmoneostramineus contained many physiologically beneficial substances for human health. Therefore, it is necessary to study the genetic diversity of Pleurotus mushroom cultivars commercially cultivated in Korea. Eleven strains of Pleurotus spp. were collected from different geographical regions in South-East Asia and ITS regions of rDNA and RAPD of genomic DNA were analyzed. The size of the ITS1 and ITS2 regions of rDNA from the different strains varied from 167 to 254 bp and 156 to 213 bp, respectively. The sequence of ITS1 was more variable than that of ITS2, and the 5.8S sequences were identical. A phylogenetic tree based on the ITS region sequences indicated that selected strains could be classified into 4 clusters. Eleven Pleurotus species were also analyzed by RAPD with 20 arbitrary primers. Ten of these primers were efficiently amplified the genomic DNA. The number of amplified bands varied with the primers and strains, with polymorphic fragments in the range from 0.1 to 2.0kb. The results revealed that genetic diversity of selected strains of P. ostreatus, P. citrinopileatus and P. salmoneostramineus is low.
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KEYWORD
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Genetic diversity ITS, Pleurotus spp. RAPD analysis
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